Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals
Identifieur interne : 000669 ( Main/Exploration ); précédent : 000668; suivant : 000670Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals
Auteurs : Basma Ouarti [États-Unis] ; Maureen Laroche [États-Unis] ; Souad Righi ; Mohamed Nadir Meguini ; Ahmed Benakhla ; Didier Raoult [États-Unis] ; Philippe Parola [États-Unis]Source :
- Parasite [ 1252-607X ] ; ????.
Descripteurs français
- KwdFr :
- Algérie (MeSH), Animaux (MeSH), Anoplura (classification), Anoplura (composition chimique), Bovins (parasitologie), Bétail (parasitologie), Capra (parasitologie), Ovis (parasitologie), Phthiraptera (classification), Phthiraptera (composition chimique), Poulets (parasitologie), Protéomique (MeSH), Pédiculoses (médecine vétérinaire), Reproductibilité des résultats (MeSH), Spectrométrie de masse MALDI (médecine vétérinaire), Volaille (parasitologie).
- MESH :
- composition chimique : Anoplura, Phthiraptera.
- médecine vétérinaire : Pédiculoses, Spectrométrie de masse MALDI.
- parasitologie : Bovins, Bétail, Capra, Ovis, Poulets, Volaille.
- Algérie, Animaux, Protéomique, Reproductibilité des résultats.
- Wicri :
- geographic : Algérie.
English descriptors
- KwdEn :
- Algeria (MeSH), Animals (MeSH), Anoplura (chemistry), Anoplura (classification), Cattle (parasitology), Chickens (parasitology), Goats (parasitology), Lice Infestations (veterinary), Livestock (parasitology), Phthiraptera (chemistry), Phthiraptera (classification), Poultry (parasitology), Proteomics (MeSH), Reproducibility of Results (MeSH), Sheep (parasitology), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (veterinary).
- MESH :
- geographic : Algeria.
- chemistry : Anoplura, Phthiraptera.
- classification : Anoplura, Phthiraptera.
- parasitology : Cattle, Chickens, Goats, Livestock, Poultry, Sheep.
- veterinary : Lice Infestations, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Animals, Proteomics, Reproducibility of Results.
Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including
Url:
DOI: 10.1051/parasite/2020026
PubMed: 32351208
PubMed Central: 7191974
Affiliations:
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Le document en format XML
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animals</title><author><name sortKey="Ouarti, Basma" sort="Ouarti, Basma" uniqKey="Ouarti B" first="Basma" last="Ouarti">Basma Ouarti</name><affiliation wicri:level="2"><nlm:aff id="AFF1"><addr-line> Aix Marseille Univ., IRD, AP-HM, SSA, VITROME 13005 Marseille France</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maine (État)</region></placeName><wicri:cityArea> Aix Marseille Univ., IRD, AP-HM, SSA</wicri:cityArea></affiliation><affiliation><nlm:aff id="AFF2"><addr-line> IHU-Méditerranée Infection 19–21 Boulevard Jean Moulin 13005 Marseille France</addr-line></nlm:aff></affiliation></author><author><name sortKey="Laroche, Maureen" sort="Laroche, Maureen" uniqKey="Laroche M" first="Maureen" last="Laroche">Maureen Laroche</name><affiliation wicri:level="2"><nlm:aff id="AFF1"><addr-line> Aix Marseille Univ., IRD, AP-HM, SSA, VITROME 13005 Marseille France</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region 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Algeria</addr-line></nlm:aff><wicri:noCountry code="subfield">Département des sciences Vétérinaire 36000 El Tarf Algeria</wicri:noCountry></affiliation><affiliation><nlm:aff id="AFF4"><addr-line> Institut des Sciences Vétérinaire et Agronomiques, Université Mohamed Cherif Messaadia 41000 Souk-Ahras Algeria</addr-line></nlm:aff><wicri:noCountry code="subfield">Université Mohamed Cherif Messaadia 41000 Souk-Ahras Algeria</wicri:noCountry></affiliation></author><author><name sortKey="Benakhla, Ahmed" sort="Benakhla, Ahmed" uniqKey="Benakhla A" first="Ahmed" last="Benakhla">Ahmed Benakhla</name><affiliation><nlm:aff id="AFF3"><addr-line> Université Chadli Bendjdid, Département des sciences Vétérinaire 36000 El Tarf Algeria</addr-line></nlm:aff><wicri:noCountry code="subfield">Département des sciences Vétérinaire 36000 El Tarf Algeria</wicri:noCountry></affiliation></author><author><name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name><affiliation><nlm:aff id="AFF2"><addr-line> IHU-Méditerranée Infection 19–21 Boulevard Jean Moulin 13005 Marseille France</addr-line></nlm:aff></affiliation><affiliation wicri:level="2"><nlm:aff id="AFF5"><addr-line> Aix Marseille Univ., IRD, AP-HM, MEPHI 13005 Marseille France</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Hawaï</region></placeName><wicri:cityArea> Aix Marseille Univ., IRD, AP-HM</wicri:cityArea></affiliation></author><author><name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name><affiliation wicri:level="2"><nlm:aff id="AFF1"><addr-line> Aix Marseille Univ., IRD, AP-HM, SSA, VITROME 13005 Marseille France</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maine (État)</region></placeName><wicri:cityArea> Aix Marseille Univ., IRD, AP-HM, SSA</wicri:cityArea></affiliation><affiliation><nlm:aff id="AFF2"><addr-line> IHU-Méditerranée Infection 19–21 Boulevard Jean Moulin 13005 Marseille France</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">Parasite</title><idno type="ISSN">1252-607X</idno><idno type="eISSN">1776-1042</idno><imprint><date when="????">????</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algeria (MeSH)</term><term>Animals (MeSH)</term><term>Anoplura (chemistry)</term><term>Anoplura (classification)</term><term>Cattle (parasitology)</term><term>Chickens (parasitology)</term><term>Goats (parasitology)</term><term>Lice Infestations (veterinary)</term><term>Livestock (parasitology)</term><term>Phthiraptera (chemistry)</term><term>Phthiraptera (classification)</term><term>Poultry (parasitology)</term><term>Proteomics (MeSH)</term><term>Reproducibility of Results (MeSH)</term><term>Sheep (parasitology)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (veterinary)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Algérie (MeSH)</term><term>Animaux (MeSH)</term><term>Anoplura (classification)</term><term>Anoplura (composition chimique)</term><term>Bovins (parasitologie)</term><term>Bétail (parasitologie)</term><term>Capra (parasitologie)</term><term>Ovis (parasitologie)</term><term>Phthiraptera (classification)</term><term>Phthiraptera (composition chimique)</term><term>Poulets (parasitologie)</term><term>Protéomique (MeSH)</term><term>Pédiculoses (médecine vétérinaire)</term><term>Reproductibilité des résultats (MeSH)</term><term>Spectrométrie de masse MALDI (médecine vétérinaire)</term><term>Volaille (parasitologie)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Algeria</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Anoplura</term><term>Phthiraptera</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Anoplura</term><term>Phthiraptera</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Anoplura</term><term>Phthiraptera</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Pédiculoses</term><term>Spectrométrie de masse MALDI</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Bovins</term><term>Bétail</term><term>Capra</term><term>Ovis</term><term>Poulets</term><term>Volaille</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Cattle</term><term>Chickens</term><term>Goats</term><term>Livestock</term><term>Poultry</term><term>Sheep</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Lice Infestations</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Proteomics</term><term>Reproducibility of Results</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Algérie</term><term>Animaux</term><term>Protéomique</term><term>Reproductibilité des résultats</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Algérie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including <italic>Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus</italic>, <italic>Menacanthus stramineus</italic>, <italic>Menopon gallinae, Chelopistes meleagridis</italic>, <italic>Goniocotes gallinae</italic>, <italic>Goniodes gigas, Lipeurus caponis</italic> and laboratory reared <italic>Pediculus humanus corporis</italic>. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Hawaï</li><li>Maine (État)</li></region></list><tree><noCountry><name sortKey="Benakhla, Ahmed" sort="Benakhla, Ahmed" uniqKey="Benakhla A" first="Ahmed" last="Benakhla">Ahmed Benakhla</name><name sortKey="Meguini, Mohamed Nadir" sort="Meguini, Mohamed Nadir" uniqKey="Meguini M" first="Mohamed Nadir" last="Meguini">Mohamed Nadir Meguini</name><name sortKey="Righi, Souad" sort="Righi, Souad" uniqKey="Righi S" first="Souad" last="Righi">Souad Righi</name></noCountry><country name="États-Unis"><region name="Maine (État)"><name sortKey="Ouarti, Basma" sort="Ouarti, Basma" uniqKey="Ouarti B" first="Basma" last="Ouarti">Basma Ouarti</name></region><name sortKey="Laroche, Maureen" sort="Laroche, Maureen" uniqKey="Laroche M" first="Maureen" last="Laroche">Maureen Laroche</name><name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name><name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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